Difference spectrophotometric method for the determination of Fluphenazine hydrochloride in tablets using peroxomonosulfate


  • Mykola Blazheyevskiy a Department of Inorganic and Physical Chemistry of National University of Pharmacy, Pushkinska street, 53, Kharkiv, Ukraine
  • Valeriy Moroz Department of Analytical Chemistry and Analytical Toxicology of National University of Pharmacy, Pushkinska street, 53, Kharkiv, Ukraine
  • Olena Mozgova a Department of Inorganic and Physical Chemistry of National University of Pharmacy, Pushkinska street, 53, Kharkiv, Ukraine http://orcid.org/0000-0003-0875-7035




spectrophotometric method, Fluphenazine hydrochloride, optical density, peroxomonosulfate


The oxidative derivatization method using potassium hydrogenperoxomonosulfate for the indirect spectrophotometric determination of Fluphenazine hydrochloride is presented. Potassium hydrogenperoxomonosulfate is introduced as a derivatizing agent for Fluphenazine hydrochloride, yielding the sulfoxide. This reaction product was successfully used for the spectrophotometric determination of the Fluphenazine hydrochloride. The UV spectroscopic detection of the sulfoxide proved to be a more robust and sensitive method. The elaborated method allowed the determination of Fluphenazine hydrochloride in the concentration range of 0.2-30 µg mL-1. The molar absorptivity at 349 nm is 5.6×103 (dm3cm-1mol-1). The limit of quantification, LOQ (10S) is 0.24 µg/mL. A new spectrophotometric technique was developed and the possibility of quantitative determination of Fluphenazine hydrochloride in tablets 5.0 mg was demonstrated. The present method is precise, accurate and excipients did not interfere. RSD for Fluphenazine Hydrochloride 5.0 mg tablets was 1.37 %.


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