Phytochemical composition, antioxidant and antiproliferative activities of Rosmarinus officinalis leaves

Winfred Nassazi, Isaac K’Owino, Jacqueline Makatiani, Sabina Wachira

Abstract

Phytochemicals in Rosmarinus officinalis leaves, their total phenolic content, antioxidant potential and antiproliferative activity against human prostate (DU145), colon (CT26) and cervical (HeLa 229) cancer cells were investigated. Extraction was done separately using hexane, dichloromethane, ethyl acetate and methanol. A total of 32 compounds were identified, eight of which were reported for the first time. The highest phenolic content was 476.80 ± 0.69 µg/ml for the methanolic extract which also had the highest antioxidant activity with a minimum inhibitory concentration of 5.39 ± 0.09 mg/ml. Extracts exhibited the highest toxicity against prostate cancer cells and the least against cervical cancer cells.

Keywords

Polyphenols, cancer, cytotoxicity, prostate adenocarcinoma

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