Phytochemical composition, antioxidant and antiproliferative activities of Rosmarinus officinalis leaves


  • Winfred Nassazi Moi University
  • Isaac K’Owino Masinde Muliro University of Science and Technology
  • Jacqueline Makatiani Moi University
  • Sabina Wachira Kenya Medical Research Institute (KEMRI), Centre for Traditional Medicine and Drug Research



Polyphenols, cancer, cytotoxicity, prostate adenocarcinoma


Phytochemicals in Rosmarinus officinalis leaves, their total phenolic content, antioxidant potential and antiproliferative activity against human prostate (DU145), colon (CT26) and cervical (HeLa 229) cancer cells were investigated. Extraction was done separately using hexane, dichloromethane, ethyl acetate and methanol. A total of 32 compounds were identified, eight of which were reported for the first time. The highest phenolic content was 476.80 ± 0.69 µg/ml for the methanolic extract which also had the highest antioxidant activity with a minimum inhibitory concentration of 5.39 ± 0.09 mg/ml. Extracts exhibited the highest toxicity against prostate cancer cells and the least against cervical cancer cells.

Author Biographies

Winfred Nassazi, Moi University

Postgraduate Researcher, Department of Chemistry and Biochemistry, Moi University, Eldoret, Kenya

Isaac K’Owino, Masinde Muliro University of Science and Technology

Isaac O. Kowino (PhD) is a Senior Chemistry Lecturer, Department of Pure and Applied Chemistry, Masinde Muliro University of Science and Technology, Kakamega, Kenya. Adjunct Chemistry Lecturer at Department of Chemistry and Biochemistry, Moi University, Eldoret, Kenya.

Jacqueline Makatiani, Moi University

Jacqueline Makatiani (PhD) is a Senior Lecturer at Department of Biological Sciences, Moi University, Eldoret, Kenya.

Sabina Wachira, Kenya Medical Research Institute (KEMRI), Centre for Traditional Medicine and Drug Research

Dr. Sabrina Wachira is a principal Reseracher at Kenya Medical Research Institute (KEMRI), Centre for Traditional Medicine and Drug Research, Nairobi, Kenya.


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