The Study of the Composition of Chloroform Fraction of Anemone nemorosa L.

Oksana Khropot, Yulian Konechnyi, Nataliya Polish, Roksolana Konechna, Yehor Bazavluk, Olena Kornіychuk, Volodymyr Novikov

Abstract

Expanding the range of herbal drugs is among the priorities of the modern pharmacy. The analysis presented in this article is drawn from a broader qualitative study examining the composition of Anemone nemorosa L. chloroform fraction. The study revealed 38 compounds, and 32 of them were identified. The investigated lipophilic extract has an antimicrobial activity both in terms of gram-positive and gram-negative microorganisms. The maximal antimicrobial action of the investigated extract was shown to Escherichia coli. The significant content of biologically active substances in the lipophilic volatile fraction of Anemone nemorosa indicates the prospects for further study.

Keywords

Anemone nemorosa; chloroform fraction; gas chromatography; lipophilic extract; antimicrobial activity

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References

Lukianchuk A, Khropot O, Konechnyi Y, Konechna R, Novikov V. Wood anemone. Anemone Nemorosa L. Analytical review. ScienceRise: Pharmaceutical Science 2017;0(3 (7)):34-38. https://doi.org/10.15587/2519-4852.2017.104438

Cumo CM. Encyclopedia of Cultivated Plants: From Acacia to Zinnia [3 Volumes]: From Acacia to Zinnia: ABC-CLIO, Santa Barbara, 1236 p. https://doi.org/10.1108/rr-01-2014-0007

Botanical.com [homepage on the Internet]. Anemone (Wood); 1995-2017. http://botanical.com

Mondoni A, Probert R, Rossi G, Hay F, Bonomi C. Habitat-correlated seed germination behaviour in populations of wood anemone (Anemone nemorosa L.) from northern Italy. Seed Science Research 2008;18(4):213-222. https://doi.org/10.1017/s0960258508084997

Bobadilla Fazzini R, Skindersoe M, Bielecki P, Puchałka J, Givskov M, Martins dos Santos V. Protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response. Environmental Microbiology 2012;15(1):111-120. https://doi.org/10.1111/j.1462-2920.2012.02792.x

Duan H, Zhang Y, Xu J, Qiao J, Suo Z, Hu G, Mu X. Effect of anemonin on NO, ET-1 and ICAM-1 production in rat intestinal microvascular endothelial cells. Journal of Ethnopharmacology 2006;104(3):362-366. https://doi.org/10.1016/j.jep.2005.09.034

Huang Y, Lee T, Chan K, Hsu F, Wu Y, Lee M. Anemonin is a natural bioactive compound that can regulate tyrosinase-related proteins and mRNA in human melanocytes. Journal of Dermatological Science 2008;49(2):115-123. https://doi.org/10.1016/j.jdermsci.2007.07.008

Jia D, Han B, Yang S, Zhao J. Anemonin Alleviates Nerve Injury After Cerebral Ischemia and Reperfusion (I/R) in Rats by Improving Antioxidant Activities and Inhibiting Apoptosis Pathway. Journal of Molecular Neuroscience 2014;53(2):271-279. https://doi.org/10.1007/s12031-013-0217-z

Lee T, Huang N, Lai T, Yang A, Wang G. Anemonin, from Clematis crassifolia, potent and selective inducible nitric oxide synthase inhibitor. Journal of Ethnopharmacology 2008;116(3):518-527. https://doi.org/10.1016/j.jep.2007.12.019

Roth L, Daunderer M, Kormann K. Giftpflanzen-Pflanzengifte, Auflage 4: Nikol Verlag, Hamburg, 1090 p.

Xiao K, Cao S, Jiao L, Lin F, Wang L, Hu C. Anemonin improves intestinal barrier restoration and influences TGF-β1 and EGFR signaling pathways in LPS-challenged piglets. Innate Immunity 2016;22(5):344-352. https://doi.org/10.1177/1753425916648223

Hao D, Gu X, Xiao P. Anemone medicinal plants: ethnopharmacology, phytochemistry and biology. Acta Pharmaceutica Sinica B 2017;7(2):146-158. https://doi.org/10.1016/j.apsb.2016.12.001

Martín M, Román L, Domínguez A. In VitroActivity of Protoanemonin, an Antifungal Agent. Planta Medica 1990;56(01):66-69. https://doi.org/10.1055/s-2006-960886

Misra S, Dixit S. Antifungal principle ofRanunculus sceleratus. Economic Botany 1980;34(4):362-367. https://doi.org/10.1007/bf02858312

Tsevegsüren N, Aitzetmüller K. γ-linolenic acid inAnemone spp. seed lipids. Lipids 1993;28(9):841-846. https://doi.org/10.1007/bf02536240

Redbook-ua.org [homepage on the Internet]. Chervona knyha Ukrayiny; 2010-2017. http://redbook-ua.org

Kozoriz O. Likarski roslyny Karpat. Dykorosli ta kulturni: Mystetska Liniya, Uzhgorod, 504 p.

Hrodzinskyi M. Likarski roslyny: Entsyklopedychnyi dovidnyk: Vydavnytstvo “Ukrayinska Entsyklopediya” im. M.P. Bazhana, Kyiv, 544 p.

Magiorakos A, Srinivasan A, Carey R, Carmeli Y, Falagas M, Giske C, Harbarth S, Hindler J, Kahlmeter G, Olsson-Liljequist B, Paterson D, Rice L, Stelling J, Struelens M, Vatopoulos A, Weber J, Monnet D. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection 2012;18(3):268-281. https://doi.org/10.1111/j.1469-0691.2011.03570.x