The quantitative determination of Perphenazine in tablets by the spectrophotometric method as its sulfoxide obtained with diperoxyazelaic acid

Mykola Blazheyevskiy, Myhailo Kucher, Oleh Shpychak


The oxidative derivatization method using diperoxyazelaic acid for the indirect spectrophotometric determination of Perphenazine dihydrochloride is presented. Diperoxyazelaic acid is introduced as a derivatizing agent for Perphenazine, yielding sulfoxides. This reaction product was successfully employed for the spectrophotometric determination of Perphenazine dihydrochloride. The UV spectroscopic detection of sulfoxide has been proven to be the more robust and selective. The method developed allowed determination of Perphenazine dihydrochloride in the concentration range of 1–40 µg/mL. The limits of quantification (LOQ=10S) is 3.3 µg·ml-1. A new spectrophotometric method has been developed, and the possibility of the quantitative determination of Perphenazine dihydrochloride in Perphenazine Tablets has been demonstrated. The present method is precise, accurate and other inactive excipients of the drug do not interfere. RSD = 2.00%; δ=( -µ) 100%/µ = – 0.85 %).


Perphenazine Dihydrochloride; Perphenazine sulfoxide; indirect spectrophotometry

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Hartung B, Sampson S, Leucht S, Hartung B, Sampson S, Leucht S. Cochrane Database of Systematic Reviews Perphenazine for schizophrenia (Review). Perphenazine for schizophrenia. Cochrane Database of Systematic Reviews 2015;3:1-447. Art. No.: CD003443. Published by John Wiley & Sons, Ltd.

Puzanowska-Tarasiewicz H, Kuźmicka L, Karpińska J, Mielech-Lukasiewicz K. Efficient Oxidizing Agents for Determination of 2,10-Disubstituted Phenothiazines. Analytical Sciences 2005;21(10):1149-1153.

Wang R, Lu Y. Kinetic spectrophotometric method for determination of perphenazine based on monitoring the oxidation intermediate by applying a stopped-flow technique. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2005;61(5):791-797.

ing Wang, Yan-Yu Xiao, Ming-Lei Chen, Fan Zeng, Li Zong. Pharmacokinetics of orally disintegrating tablets of perphenazine/hydroxypropyl-cyclodextrin inclusion complex in rabbits. Pharmazie 2013; 68(10):800–804

Saar E, Gerostamoulos D, Drummer O, Beyer J. Identification and quantification of 30 antipsychotics in blood using LC-MS/MS. Journal of Mass Spectrometry 2010;45(8):915-925.

Turunen E, Lehtonen M, Järvinen T, Jarho P. Development and validation of a gas chromatographic–mass spectrometric method for quantitative determination of perphenazine in rabbit plasma after sublingual administration. Journal of Chromatography B 2008;872(1-2):51-57.

Patel, D., & Patel, V. Simultaneous estimation of Amitriptyline Hydrochloride and Perphenazine by Absorption Ratio (Q-analysis) UV Spectrophotometric method in combined tablet dosage form. International journal of pharmaceutical sciences and research 2010;1(12):.

Guo L, Zhang Y, Li Q. Spectrophotometric determination of perphenazine with the detection system of potassium ferricyanide–Fe(III) in pharmaceutical and serum samples. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2009;74(2):307-311.

Heli H, Sattarahmady N, Zare S. Electrooxidation and determination of perphenazine on a graphene oxide nanosheet-modified electrode. RSC Advances 2015;5(27):21005-21011.

Fasihi F, Farjami F, Shafiee G. Highly sensitive determination of perphenazine on a carbon nanocomposite ionic liquid electrode. RSC Advances 2015;5(115):95087-95095.

Cşofreţ V, Buck R. Organics. Pharmaceutical Applications of Membrane Sensors 2018;:111-380.

Tu Y, Hsieh M, Chang S. Sensitive detection of piperazinyl phenothiazine drugs by field-amplified sample stacking in capillary electrophoresis with dispersive liquid-liquid microextraction. ELECTROPHORESIS 2015;36(21-22):2828-2836.

Stolman A. Progress in Chemical Toxicology Vol. 5 / Ed. Abraham Stolman. Elsevier, 2013:406. ISBN: 9781483219233

Rezaei B, Mokhtari A. Sensitive determination of perphenazine in pharmaceuticals and human serum by flow injection chemiluminescence method using [Ru(phen)3]2+-ce(IV) system and a chemometrical optimization approach. Journal of the Brazilian Chemical Society 2011;22(1):49-57.

The British Pharmacopoeia Commission Secretariat of the Medicines and Healthcare Products Regulatory Agency (MHRA) The British Pharmacopoeia 2019. London, United Kingdom: TSO (The Stationery Office); 1 January 2019; Available from

The United States Pharmacopeia, Thirty-Ninth Revision, and The National Formulary, Thirty-Fourth Edition. The United States Pharmacopeial Convention 12601 Twinbrook Parkway, Rockville, MD 20852

Shlusar, O. E., & Blazheevski, M. Y.. Spectrophotometric determination of perphenazine as it’s sulphoxide obtained by peroxomonosulphate. Farmatsevtychnyi Zhurnal, 2012; 4: 71-75 (Ukrainian). Retrieved from

Blazheyevskiy M.Ye. Application of derivatization by means of peroxy acid oxidation and perhydrolysis reactions in pharmaceutical analysis : Monograph – Lviv: Ivan Franko National University of Lviv, 2017:106.