The quantitative determination of Perphenazine in tablets by the spectrophotometric method as its sulfoxide obtained with diperoxyazelaic acid

Mykola Blazheyevskiy; Myhailo Kucher; Oleh Shpychak

doi:10.17721/fujcV7I2P96-103

Authors

Mykola Blazheyevskiy
Myhailo Kucher
Oleh Shpychak Oleg S. Shpychak (Shpychak O. S.). – corresponding author Doctor of Pharmacy, Professor, Head of the Department of Industrial Pharmacy and Economy Institute for Advanced Training of Pharmacy Specialists (IATPS) of the National Pharmaceutical University, Kharkiv, Ukraine. http://orcid.org/0000-0002-3015-8584

DOI:

https://doi.org/10.17721/fujcV7I2P96-103

Keywords:

Perphenazine Dihydrochloride, Perphenazine sulfoxide, indirect spectrophotometry

Abstract

The oxidative derivatization method using diperoxyazelaic acid for the indirect spectrophotometric determination of Perphenazine dihydrochloride is presented. Diperoxyazelaic acid is introduced as a derivatizing agent for Perphenazine, yielding sulfoxides. This reaction product was successfully employed for the spectrophotometric determination of Perphenazine dihydrochloride. The UV spectroscopic detection of sulfoxide has been proven to be the more robust and selective. The method developed allowed determination of Perphenazine dihydrochloride in the concentration range of 1–40 µg/mL. The limits of quantification (LOQ=10S) is 3.3 µg·ml-1. A new spectrophotometric method has been developed, and the possibility of the quantitative determination of Perphenazine dihydrochloride in Perphenazine Tablets has been demonstrated. The present method is precise, accurate and other inactive excipients of the drug do not interfere. RSD = 2.00%; δ=( -µ) 100%/µ = – 0.85 %).

Author Biography

Oleh Shpychak, Oleg S. Shpychak (Shpychak O. S.). – corresponding author Doctor of Pharmacy, Professor, Head of the Department of Industrial Pharmacy and Economy Institute for Advanced Training of Pharmacy Specialists (IATPS) of the National Pharmaceutical University, Kharkiv, Ukraine.

Doctor of Pharmacy, Professor, Head of the Department of Industrial Pharmacy and Economy Institute for Advanced Training of Pharmacy Specialists (IATPS) of the National Pharmaceutical University, Kharkiv, Ukraine.

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