A new oxidative derivatization method for spectrophotometric determination of Periciazine in pharmaceutical preparations

Mykola Blazheyevskiy, Valeriy Moroz


A new the oxidative derivatization method by means of peroxoacid for the indirect spectrophotometric determination of Periciazine is presented. A potassium hydrogenperoxymonosulfateas a derivatizing agent for Periciazine, yielding the absorbative Periciazine sulfoxide at λmaх=362 nm is proposed. This reaction product was successfully employed for spectrophotometric determination of the Periciazine. The UV spectrophotometric determination of the Periciazine as its sulfoxide proved to be the more simple and selective method. Limit of quantification (LOQ=10S) is 2.8 µg·mL-1. The common excipients employed do not interfere in the determination of phenothiazine drug. Results of analysis of the drug dosage forms by the proposed method are in good agreement with those of the official method. RSD=1.76 % (δ <RSD).


Spectrophotometry; oxone; Periciazine, phenothiazine sulphoxide; pharmaceutical preparations

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Danilov DS. Periciazine «forgotten» by researchers and its significance for modern clinical practice. Psychiatry and psichopharmacotherapy. 2015; 1: 31–37. [In Russ.].

Bekker RA., Bykov YuV. Pericyazine and its clinical used. Psychiatry and Psychopharmacotherapy. 2018; 20 (2):16–31 [In Russ.].

Sajjan AG, Ramesh KC, Jaldappagari Seetharamappa, Keshavayya J. Spectrophotometric investigations on the assay of phenothiazine drugs. Indian J Pharm Sci. 2002 Jun; 64(6): 603-606.

Puzanowska-Tarasiewicz H, Karpińska J, Kuźmicka L. Analytical Applications of Reactions of Iron(III) and Hexacyanoferrate(III) with 2,10-Disubstituted Phenothiazines. International Journal of Analytical Chemistry 2009;2009:1-8. https://doi.org/10.1155/2009/302696

Mielech‐Łukasiewicz K, Puzanowska‐Tarasiewicz H, Panuszko A. Electrochemical Oxidation of Phenothiazine Derivatives at Glassy Carbon Electrodes and Their Differential Pulse and Square‐wave Voltammetric Determination in Pharmaceuticals. Analytical Letters 2008;41(5):789-805. https://doi.org/10.1080/00032710801935038

Lux M, Vigneron J, Perrin A, Hoffman MA, Hoffman M. Refractometric Screening of Oral Syringes. Can. J Hosp. Pharm. 2018 Aug;48(5). https://doi.org/10.4212/cjhp.v48i5.2324

Tanaka E, Nakamura T, Terada M, Shinozuka T, Hashimoto C, Kurihara K, Honda K. Simple and simultaneous determination for 12 phenothiazines in human serum by reversed-phase high-performance liquid chromatography. Journal of Chromatography B 2007;854(1-2):116-120. https://doi.org/10.1016/j.jchromb.2007.04.004

Cai H, Deng Y, Fang P, Cao S, Hou Z, Wu Y, Chen X, Yan M, Zhang B. A sensitive LC–MS/MS method for analysis of pericyazine in presence of 7-hydroxypericyazine and pericyazine sulphoxide in human plasma and its application to a comparative bioequivalence study in Chinese healthy volunteers. Journal of Pharmaceutical and Biomedical Analysis 2017;135:67-74. https://doi.org/10.1016/j.jpba.2016.12.007

Temerdashev Z, Kiseleva N, Klishchenko R, Udalov A. Separation and identification of phenothiazine compounds by thin-layer chromatography. Journal of Analytical Chemistry 2006;61(1):2-5. https://doi.org/10.1134/s1061934806010023

Klishchenko RA, Temerdashev ZA, Kiseleva N.V. Peculiarities of TLC screening of phenothiazine derivatives. Bulletin of universities. North Caucasian region. Natural Sciences. 2005; 3:59-62 [in Russ.].

The United States Pharmacopeia, Thirty-Ninth Revision, and The National Formulary, Thirty-Fourth Edition. The United States Pharmacopeial Convention 12601 Twinbrook Parkway, Rockville, MD 20852

European Pharmacopoeia 9th Edition – European Directorate for the Quality of Medicines (EDQM) – Council of Europe, 67075 Strasbourg Cedex, France 2016. 4016 p.

Blazheyevskiy M.Ye. Application of derivatization by means of peroxy acid oxidation and perhydrolysis reactions in pharmaceutical analysis. Lviv: Ivan Franko National University of Lviv; 2017.